Table 1 Primers and their sequences used... | Emerald Publishing

Table 1

Primers and their sequences used in amplification of the product

Primer name	Sequence 5′–3′	No. of bases	Product sizes	Melting temp.
FR8/9-Forward	ACCTCACCCTGTGGAGCCAC	20	214	64.8
FR8/9-Reverse M	CCTTGCCCCACAGGGCAGTAACGGCACACC	30	214	74.8
FR41/42-Forward	ACCTCACCCTGTGGAGCCAC	20	448	64.8
FR41/42-Reverse M	GAGTGGACAGATCCCCAAAGGACTCAACCT	30	448	67.8
IVS1G > T-Forward	ACCTCACCCTGTGGAGCCAC	20	281	64.8
IVS1G > T-Reverse M	TTAAACCTGTCTTGTAACCTTGATACGAAA	28	281	56.4
IVS 1-5 G > C-Forward	ACCTCACCCTGTGGAGCCAC	20	285	64.8
IVS 1-5 G > C-Reverse M	CTCCTTAAACCTGTCTTGTAACCTTGTTAG	28	285	58.5
CAP+1 A > G -Forward	AAAAGTCAGGGCAGAGCCATCTATTGGTTC	24	596	66.6
CAP+1 A > G -Reverse M	CCCCTTCCTATGACATGAACTTAA	30	596	60.6

Primer name	Sequence 5′–3′	No. of bases	Product sizes	Melting temp.
FR8/9-Forward	ACCTCACCCTGTGGAGCCAC	20	214	64.8
FR8/9-Reverse M	CCTTGCCCCACAGGGCAGTAACGGCACACC	30	214	74.8
FR41/42-Forward	ACCTCACCCTGTGGAGCCAC	20	448	64.8
FR41/42-Reverse M	GAGTGGACAGATCCCCAAAGGACTCAACCT	30	448	67.8
IVS1G > T-Forward	ACCTCACCCTGTGGAGCCAC	20	281	64.8
IVS1G > T-Reverse M	TTAAACCTGTCTTGTAACCTTGATACGAAA	28	281	56.4
IVS 1-5 G > C-Forward	ACCTCACCCTGTGGAGCCAC	20	285	64.8
IVS 1-5 G > C-Reverse M	CTCCTTAAACCTGTCTTGTAACCTTGTTAG	28	285	58.5
CAP+1 A > G -Forward	AAAAGTCAGGGCAGAGCCATCTATTGGTTC	24	596	66.6
CAP+1 A > G -Reverse M	CCCCTTCCTATGACATGAACTTAA	30	596	60.6

Source(s): By authors (Ms Ayesha Ghalib under the supervision of Dr Waleed)