A line graph showing FSign2 as a function of Cai. The x-axis represents Cai ranging from 0.0 to 1.0, and the y-axis represents FSign2 ranging from 0 to 12. The graph features a single red line that starts at a high value, dips to a minimum around Cai equals 0.6, and then rises again. There is also a horizontal blue line at FSign2 equals 0. All values are approximated.as a function of with , and . Note that we have assumed that . It can happen that for one root in and that for the other root in in which case only for . The results still hold. If for both roots, then in the feasible range of . Again, the results will hold
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