This study introduces a novel bio-nano soil stabilisation technique that combines microbially induced calcium carbonate precipitation (MICP) with amorphous nano-silica (NS) to improve the geotechnical properties of various sandy soils. The research aims to enhance soil strength and sustainability while evaluating the environmental trade-offs. Laboratory experiments were conducted on medium-to-fine sand, silty sand, and clayey sand, investigating the influence of bacterial concentration (101–107 cfu/ml), cementation solution molarity (0.25–2.0 M), and NS dosage (1%–2.5% by weight). Key performance indicators included compaction characteristics, permeability, California Bearing Ratio (CBR), and ultrasonic pulse velocity (UPV). Under optimal conditions (106 cfu/ml and 1.75 M solution), the treatment significantly improved CBR and UPV while reducing permeability. The addition of 2% NS further enhanced performance, increasing dry density and CBR by up to 73% and 131%, respectively, while reducing permeability by 57%. X-ray diffraction confirmed calcite formation, validating successful mineralisation. A cradle-to-gate life cycle assessment revealed a 49% increase in carbon footprint due to NS use, indicating a trade-off between performance gains and environmental impact. These findings suggest a promising, synergistic solution for sustainable ground improvement.
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15 April 2026
Research Article|
December 16 2025
Bio-nano soil stabilisation using MICP and nano-silica: performance and sustainability
Sourya Snigdha Mohapatra;
Department of Civil Engineering,
Veer Surendra Sai University of Technology (VSSUT)
, Sambalpur, India
Corresponding author Sourya Snigdha Mohapatra (ssmohapatra_phdce@vssut.ac.in)
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Rakesh Roshan Dash;
Rakesh Roshan Dash
Department of Civil Engineering,
Veer Surendra Sai University of Technology (VSSUT)
, Sambalpur, India
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Pradip Kumar Pradhan
Pradip Kumar Pradhan
Department of Civil Engineering,
Veer Surendra Sai University of Technology (VSSUT)
, Sambalpur, India
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Corresponding author Sourya Snigdha Mohapatra (ssmohapatra_phdce@vssut.ac.in)
Disclosures No potential conflict of interest was reported by the authors.
Publisher: Emerald Publishing
Received:
June 06 2025
Accepted:
November 10 2025
Online ISSN: 2051-803X
© 2025 Emerald Publishing Limited
2025
Emerald Publishing Limited
Licensed re-use rights only
Environmental Geotechnics (2026) 13 (3): 199–222.
Article history
Received:
June 06 2025
Accepted:
November 10 2025
Citation
Mohapatra SS, Dash RR, Pradhan PK (2026), "Bio-nano soil stabilisation using MICP and nano-silica: performance and sustainability". Environmental Geotechnics, Vol. 13 No. 3 pp. 199–222, doi: https://doi.org/10.1680/jenge.25.00107
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