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The impact of varying flexural reinforcement ratios in the orthogonal directions (⁠ρx and ρy⁠) on the punching shear resistance (PSR) of flat slabs was experimentally investigated. Six slabs, measuring 1.5 m × 1.5 m × 0.15 m, were tested, with a constant average reinforcement ratio (⁠ρav⁠) of 0.3% and ρx/ρy ratios ranging from 1.2 to 3.0. The results indicated an insignificant effect on punching resistance for ρx/ρy ≤ 2.5. Increasing ρx/ρy led to wider, more dispersed cracks and enhanced ductility. Comparisons of various codes (BS 8110-1:1997, ACI 318-19, the first and second generations of Eurocode 2 (EC2-2004 and EC2-2023, respectively) and fib Model Code 2010 (MC2010)) revealed that BS 8110-1:1997 provided conservative predictions except for ρx/ρy = 3.0, while ACI 318-19 consistently overestimated the punching resistance. MC2010 (level of approximation IV) accounted for the reduction in PSR at higher ρx/ρy ratios. Tests from the literature with ρx/ρy up to 4.4 exhibited further strength reduction. Comparisons of EC2-2004 and EC2-2023 showed that EC2-2004 gave conservative estimates, with diminishing accuracy as ρx/ρy increased, while EC2-2023 accurately predicted the punching resistance for ρx/ρy ≤ 2.5, but overestimated it for higher ratios. This study highlights the limitations of current codes in addressing reinforcement asymmetry effects, and recommends incorporating a reduction factor in EC2-2023 for ρx/ρy > 2.5 to account for reduced PSR.

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