Corrigendum Free

Effect of silicate nanoplatelets on hMSC differentiation. (a) The addition of silicate nanoplatelets upregulate the ALP activity of hMSCs. (b) The increase in the RUNX2 (green) and production of bone-related proteins, such as osteocalcin (OCN, green) and osteopontin (OPN, red), was observed due to the addition of silicates. Cells in normal media without silicate particles act as a negative control, whereas cells in an osteoinductive medium serve as a positive control. Cell nuclei were counterstained with 40,6-diamidino-2-phenylindole (DAPI) (blue). (c) The protein production was quantified using image analysis of the fluorescence images. The intensity of protein per cell was quantified and later normalized by the control (hMSCs in normal growth media with no silicate particles) to obtain a fold-increase in the production of protein. OC, osteoconductive; OI, osteoinductive (from Gaharwar et al.⁷³)