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The disinfecting ability of hibiscus seed proteins against Escherichia coli, total coliforms and faecal coliforms in water treatment was investigated. Hibiscus seeds are widely available as a primary source of protein in tropical countries. Using varying doses of extracts, partial inactivation of faecal coliforms and E. coli was achieved. Hibiscus sabdariffa crude extract (SCE) achieved 67% faecal coliform inactivation whereas Hibiscus cannabinus (kenaf) crude extract (KCE) achieved 57% using dosages of 200 mg/l. Additionally, E. coli were observed to be more sensitive to KCE than SCE, with maximum inactivations of 65% and 60%, respectively. Furthermore, complete bacterial regrowth was witnessed with the extracts after 72 h storage time. However, purified sabdariffa protein (PSP) and purified kenaf protein (PKP) showed complete (100%) bacterial inactivation post-treatment, using a dose of 1·48 mg/l. Additionally, PSP and PKP achieved 92% and 90% turbidity removal, respectively, from a value of 11·9 NTU. Therefore, the use of PSP and PKP in water treatment could improve access to clean water in developing countries.

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