In view of the current difficulty of separation of troxerutin, the purpose of the paper is to separate and purify semi-synthetic flavonoid compound troxerutin by macroporous adsorption resin (SZ-3).
Comparing the adsorption performance and resolution of three different polar resins and choosing a resin to optimize the process parameters such as sample volume, eluent concentration and elution temperature to obtain high-purity troxerutin. After separating and enriching by resin column chromatography, detected the sample by LC-MS analysis.
This research found that the optimal conditions of the adsorption and desorption were sample volume S = 90 mg/g resin, methanol concentration C = 25%, T = 20 °C. the content of troxerutin increased significantly from 88% to more than 96%. Then confirmed the sample was troxerutin by LC-MS. In addition, the resin could be used for at least 10 cycles in the separation and purification experiments of troxerutin.
Purification of troxerutin with new SZ-3 resin for the first time. Under the optimal conditions, the purity and recovery of troxerutin was 96.4% and 39%. In this study, the authors established a purification process of troxerutin successfully that was simple, economical, environment friendly, with high purity and high recovery rate to provide a reference program for changing the status of troxerutin separation difficulties.
