Embryonic Stem Cells: Methods and Protocols

This volume provides 35 chapters containing step‐by‐step protocols used in various embryonic stem (ES) cell technologies. Over 90 scientists from the UK, Canada, Australia, France, Germany, Italy, Switzerland, and Japan, have contributed to this volume and the editor believes it will be useful to both experts and novices in ES cell technology, and, hopefully, will lead to further developments.

The book begins with protocols for ES cell isolation, maintenance, and modulation of gene expression, together with studies of ES cell cycle and apoptosis. There are chapters on protocols for the use of ES cells to generate tissue types, such as...