Comparing dry and wet chemical techniques for carbon stock assessment in senescent litter Available to Purchase

Accurate quantification of carbon stocks in ecosystems is essential for effective climate change mitigation. This study evaluates three common methods for measuring carbon content in senescent litter to identify the most reliable and practical approach: wet combustion (Walkley–Black (WB)) and two forms of dry combustion (muffle furnace incineration (MUFLA) and elemental analysis (CNHS)). For this purpose, monthly litter samples (leaves, branches, and miscellaneous) were collected over a year in a moist forest refuge in Chapada do Araripe, Brazil. Statistical variance analysis revealed significant differences among methods, with dry combustion techniques consistently yielding higher carbon values (MUFLA > CNHS > WB). The WB method also produced less reliable results and still requires hazardous chemicals for analysis, making its replacement by dry combustion preferable. Given its simplicity, safety, and effectiveness, MUFLA may be the most appropriate method for researchers assessing carbon stocks in senescent litter to estimate CO₂ sequestration rates and support climate mitigation strategies.