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Crack repair in concrete is crucial, since cracks are the main cause of decreased service life in concrete structures. An original and promising way to repair cracks is to pre-incorporate healing agents inside the concrete matrix to heal cracks the moment they appear. By incorporating bacteria and nutrients as a two-component healing agent, the process of bacterially mediated calcium carbonate deposition is triggered on crack formation, and self-healing of cracks can be expected. This paper investigates the recovery of mechanical properties due to self-healing behaviour of bacteria-based engineered cementitious composites (ECCs). In this research study, Sporosarcina pasteurii and Bacillus subtilis subsp. spizizenii were selected as two different bacterial strains, with zeolite as a protective carrier. Four-point bending and ultrasonic pulse velocity tests were performed on ECC specimens to evaluate their mechanical properties during damage and healing processes. Microstructural observations to quantify self-healing compounds were performed using X-ray diffraction analyses and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. The bacteria-incorporated ECC specimens were found to be promising in the healing of cracks (showing total healing of 80 μm wide cracks) and recovery of flexural strength and stiffness.

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